Submission of NMR samples
The NMR facility is a central facility and is open to all users of the department of chemistry. Users are members of scientific working groups or students who carry out NMR measurements during lab courses. External measurements can be carried out on request to the scientific heads of the NMR department. Costs for such measurements can also be requested.
Below you will find our request forms and information on the measurements. Please use only the current request forms!
- The length of the tubes (5 mm and 10 mm) must be at least 16.5 cm and at most 18 cm.
- The filling height must not be less than 4.5 cm and should not be more than 6 cm.
- Deuterated solvents must be used (enter on the request form).
- The sample must be completely dissolved, so that the solution is homogeneous liquid with low viscosity.
- In general, you should give as much sample as you can. Doubling the sample quantity reduces the measuring time by a factor of 4!
- Further instructions for filling out the request forms
Please remember to pick up measured NMR samples immediately! The samples are sorted according to the working groups, so that the samples of other group members can be taken along. NMR samples that have not been collected are disposed of at regular intervals!
Standard and Polymer Samples
A standard sample is a solution NMR sample of a non-air-sensitive and non-paramagnetic substance for the measurement of routine spectra. Routine spectra are 1D 1H, 13C, 19F, 31P measurements and 2D HH-COSY, HH-TOCSY, HSQC, HMBC, NOESY spectra. These experiments can be measured with or without water suppression. On request, samples in 3 mm tubes can also be measured.
- To submit your samples for NMR measurements, please, place your samples in room OC-021 with request forms.
- The minimum quantity to be supplied is 1 mg. When weighing in, remember that the sample may contain secondary components and salts.
- Each sample submitted for routine service for 1D-13C and 2D experiments must be checked for cleanliness/quantity with a 1H spectrum beforehand. If this rule is disregarded, the NMR service reserves the right to temporarily exclude you from the service. (The measurement of a 1H spectrum of 2 mg substance takes 1 minute, of a 13C spectrum approx. 1 hour).
- Before measuring samples on the 600 MHz spectrometer, we also ask you to record a 1H spectrum on one of the other spectrometers. We retain the option to have the 1H spectrum shown to us before the measurement.
For the measurement of polymers, please select "polymer sample" on the request form, as we may use different experimental parameters. For requests regarding the consideration of relaxation times, please note this on the form.
Please make an appointment for air-sensitive, light-sensitive or otherwise unusual samples (nmr"AT"chemie.uni-hamburg.de), so that we can perform the measurement quickly after delivery. Measurements at different temperatures can only be made by appointment and, depending on the effort involved, we may need a few days' advance notice.
Open Access Spectrometer
Please use only the form at the end of this section for samples that you measure on the Open Access device. Important: Please follow these instructions for the identification of your data on the file server:
The experiment name always has the form: Date-F300UHH. As title, please enter an identifier in the first line: INSTITUTE-WORKGROUP-NAME. The abbreviations for Institute and WORKGROUP/lab course can be found on the sample sheet. NAME is your department identifier (obtained by IT Service). You can write your sample name in the second line of the title.
In case of failure to do so, the data cannot be assigned to the right directory at first, so that it will take longer until the data are accessible to you.
Lab course (ISP): Please enter PRA-ISP5-COURSENUMBER in the first line of the title.
- The measurements are done in room AC-019 (computer on the right).
- The minimum sample quantity for 1D-13C and 2D experiments is 10 mg.
- 1H-NMR measurements to check the sample required beforehand to request other NMR service can be performed on the Fourier 300.
- Follow the instructions in the sampling room. As this is a room for doing NMR experiments and not a laboratory, wearing lab coats, gloves and other laboratory equipment is prohibited.
- Please retrieve your samples within 1 day so that other users can also use the Fourier 300. Since we share the instrument, other people will be just as happy as you to find a free slot! Please inform the NMR staff if you cannot retrieve your samples due to illness etc.
- The NMR staff empties the sample changer once per week. The samples are dispensed in room OC-021 (sample dispensing room). Please do not place samples in AC-019.
- It is forbidden to collect other’s samples!!! You should collect your own sample only.
- In case of faults on the device, please inform the NMR staff (email@example.com).
Measurements at Different Temperatures
Measurements at different temperatures can only be made by appointment and, depending on the effort involved, we may need a few days' advance notice.. Please send your request to: (nmr"AT"chemie.uni-hamburg.de)
Solid State NMR
Please send your request for solid state NMR to: nmr"AT"chemie.uni-hamburg.de