4. Daniel Wilson - Dynamic interplay between chloramphenicol/linezolid and the translating ribosome

This project addresses the dynamic interplay between antibiotics and the translational apparatus. We will address the mechanism of ribosome inhibition and resistance to chloramphenicol and oxazolidinone antibiotics. Specifically, the mechanism by which these drugs interplay with the nascent polypeptide chain to mediate ribosome stalling, and thereby induce expression of downstream resistance genes will be investigated. Additionally, biochemical studies addressing the mechanism of proline stalling will be continued. Collectively, such studies provide mechanistic insight into how diverse ribosomal ligands interplay with the ribosome to modulate the activity of the translational apparatus.