Here we perform enzymatic synthesis employing efficiently available enzymes of the carbohydrate metabolism. In contrast to the analytical biochemical approach focus is rather on the preparative aspect. With much success hydrolases can be used for synthesis in the sense of transglycosylations. Also glycosyltransferases could be employed most efficiently giving rise to complex heterooligosaccharides . Recently some focus was on an investigation of the enzyme binding and inhibition of Trypanosoma cruzi trans-sialidase(TcTS). The novel phenyl-propyl a-C-glycoside of neuraminic acid proved to be the first carbohydrate-based modulator of this enzyme as shown in Fig.2 [3,4].
Figure 2: Transglycosylation progress monitored by NMR .
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