We analyze by high field NMR spectroscopy the structure of oligosaccharides that are present in glycoconjugates. This includes the primary structure and the three dimensional structure. Mainly, NMR spectroscopy and mass spectronomy is being used for these types of analyses. It is paramount to characterize even minor amounts of oligosaccharides on glycoproteins to understand their function in the biological system. Presence of immunogenic or structures that influence the clearance of glycoproteins from the body is very important. Also, the structure of oligosaccharides, which influence uptake of glycoproteins or that affect cell-cell interactions, is important for the function of glycoproteins.
Characterization of picomole amounts of oligosaccharides from
by 1H NMR spectroscopy.
Fellenberg, M.; Coksezen, A.;
Angew Chem Int Ed
Engl 2010, 49, (14), 2630-3.
Identification of the 1H-NMR Spectra of Complex Oligosaccharides with Artificial Neural Networks.
B. Meyer, T. Hansen, D. Nute, P. Albersheim, A. Darvill, W. York & J. Sellers,
Science 251, 542-544 (1991).
Sensitivity Enhancement by Artificial Neural Network Based Recognition of 1H-NMR Spectra. Application to N-Type Oligosaccharides from Glycoproteins.
J.P. Radomski, H. van Halbeek & B. Meyer,
Nature Struct. Biol. 1, 217-218 (1994).
The V3-directed immune response in natural human immunodeficiency virus type 1 infection is predominantly directed against a variable, discontinuous epitope presented by the gp120 V3 domain.
M. Schreiber, C. Wachsmuth, H. Müller, S. Odemuyiwa, H. Schmitz, S. Meyer, B. Meyer, J. Schneider-Mergener,
J. Virol. 71, 9198-9205 (1997).
Stabilization of the Peptide Backbone through Interactions with 12 O-Glycans and one N-Glycan: NMR study of the T1 Fragment of Glycophorin AN,
J. Pieper, K.H. Ott & B. Meyer,
Nature Struct. Biol. 3, 228-232 (1996).
‘Wave-type’ structure of a synthetic hexaglycosylated decapeptide: A part of the extracellular domain of human glycophorin A.
Oliver Schuster, Gunther Klich, Volker Sinnwell, Helge Kränz, Hans Paulsen & Bernd Meyer,
J. Biomolecular NMR, 14, 33–45, (1999).