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Universität Hamburg Fachbereich Chemie
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Dr. Dessi Georgieva

Abteilung für Biochemie und Molekularbiologie
Martin-Luther-King Platz 6 - 20146 Hamburg

Telefon: 040 / 8998 - 4749, Fax: - 4747
E-Mail: Georgieva@unisgi1.desy.de

Applikationslabor am DESY
Geb. 22a, Raum 102
Notkestr. 85
22603 Hamburg


Wissenschaftlicher Werdegang:


Nationales Gymnasium der Naturwissenschaften und Mathematik (1984-1988); Studiengang Chemieingenieurwesen, Technische Universität Sofia, Abteilung für Organische Chemie (1988-1993); Promotion am Institut für Mikrobiologie, Bulgarische Akademie der Wissenschaften, Sofia (1997-2000); Wissenschaftliche Mitarbeiterin am Institut für Organische Chemie, Bulgarische Akademie der Wissenschaften, Sofia (1992-2001);  Wissenschaftliche Mitarbeiterin am Universitätsklinikum Hamburg Eppendorf / am Institut für Biochemie und Molekularbiologie der Universität Hamburg (seit 2001).


Teinahme an internationalen Projekten:


2008-2011: „Applied Venom Proteomics / Venomics“ (DFG).

2007-2010: „OptiCryst“ – Optimization of Protein Crystallization for European Structural  Genomics”. Sixth Framework Programme of the European Commision. 

2002-2003: „Isolation, crystallization and X-ray structure determination of neurotoxins from the venom of the snakes Vipera ammodytes, the most toxic snakes in Europe". Stipendium der Alexander von Humboldt-Stiftung am Universitätsklinikum Hamburg-Eppendorf.

2001–2003: “Structure-function analysis of selected snake venom proteins and application of the results for a drug design” (DFG).

2001–2002: “Structure-function relationships in hemocyanins” (NATO).

2001: “X-ray investigations of structure-function relationships in the neurotoxin Vipoxin from the venom of Vipera ammodytes meridionalis, the most toxic snake in Europe” (DAAD).

1998–2002: “Structure, function and medical application of hemocyanins” (BMBF).

1996–1997: “Dioxygen – transporting proteins in invertebrates” (Volkswagen Stiftung).


Publikationen


Buchbeiträge


Ch. Betzel, T.P. Singh, D. Georgieva and N. Genov, “Phospholipase A2”. Handbook of Metalloproteins (Messerschmidt, A., Bode, W. and Cygler, M., eds.) Vol. 3, John Wiley & Sons, Ltd, Chichester, pp. 677-691 (2004).

Reviews

D. Georgieva, R.K. Arni and Ch. Betzel, “Proteome Analysis of Snake Venom Toxins: Pharmacological Insights”. Exp. Rev. Proteomics, in press (2008).

Ch. Betzel, D. Georgieva, M. Murakami, R.K. Arni and N. Genov, “Phospholipase A2 as a biomarker of pathophysiological proceses”. Curr. Topics Biochem. Res. 8:87-93 (2006).

Publikationen

  1. D. Georgieva, A. Kardas, F. Buck. M. Perbandt and Ch. Betzel, “Isolation, Crystallization and Preliminary X-Ray Diffraction Analysis of L-Amino Acid Oxidase from Vipera ammodytes ammodytes Venom”. Acta Cryst. F 64, 918-921 (2008).

  2. D. Georgieva, M. Risch, A. Kardas, F. Buck, M. von Bergen and Ch. Betzel, „Comparative Analysis of the Venom Proteomes of Vipera ammodytes ammodytes and Vipera ammodytes meridionalis”. J. Proteome Research 7(3), 866-886 (2008). 

  3. L. Redecke, M. von Bergen, J. Clos, P.V. Konarev, D.I. Svergun, J.A. Broekaert, O. Bruns, D. Georgieva, E. Mandelkow, N. Genov and Ch. Betzel, „Structural Characterization of Beta-Sheeted Oligomers Formed on the Pathway of Oxidative Prion Protein Aggregation in vitro”. J. Struct. Biol. 157(2), 308-20 (2007).

  4. D. Georgieva, D. Schwark, M. von Bergen, L. Redecke, N. Genov and Ch. Betzel, “Interactions of Recombinant Human Prions with Compounds of Therapeutical Significance”. Biochem. Biophys. Res. Commun. 344(2), 463-70 (2006).

  5. D. Georgieva, N. Genov, W. Voelter and Ch. Betzel, “Catalytic Efficiencies of Alkaline Proteinases from Microorganisms”. Z. f. Naturforsch. C 61(5-6), 445-52 (2006).

  6. A. Petersen, R. Suck, B. Lindner, D. Georgieva, M. Ernst, H. Notbohm, D. Wicklein, O. Cromwell and W.-M. Becker, “Phl p 3: Structural and immunological characterization of a major allergen of timothy grass pollen”. Clin. Exp. Allergy 36(6), 840-9 (2006).

  7. D. Georgieva, N. Genov, W. Rypniewski, H. Echner, W. Voelter and Ch. Betzel, “Crystallization and preliminary X-ray diffraction studies show that chicken prion protein repeat binds to the proteinase K active site”. Submitted to Acta Cryst F (2005).

  8. L. Redecke, W. Meyer-Klaucke, M. Koker, J. Clos, D. Georgieva, N. Genov, H. Echner, H. Kalbacher, M. Perbandt, R. Bredehorst, W. Voelter and Ch. Betzel, “Comparative analysis of the human and chicken PrP copper binding region at pH 6.5”. J. Biol. Chem. 280, 13987 - 13992 (2005).

  9. D. Georgieva, N. Genov and Ch. Betzel, “Bacillus licheniformis variant DY proteinase: specificity in relation to the geometry of the substrate recognition site”. Curr. Microbiol. 51, 71-74 (2005).

  10. D. Georgieva, D. Schwark, P. Nikolov, K. Idakieva, K. Parvanova, K. Dierks, N. Genov and Ch. Betzel, “Conformational states of the Rapana thomasiana hemocyanin and its substructures studied by dynamic light scattering and time resolved fluorescence spectroscopy”. Biophys. J. 88 (2) 1276 - 1282 (2005).

  11. D. Georgieva, W. Rypniewski, H. Echner, M. Perbandt, M. Koker, J. Clos, L. Redecke, R. Bredehorst, W. Voelter, N. Genov and Ch. Betzel, “Synthetic human prion protein octapeptide repeat binds to the proteinase K active site”. Biochem. Biophys. Res. Commun. 325, 1406-1411 (2004).

  12. D. Georgieva, M. Koker, L. Redecke, M. Perbandt, J. Clos, R. Bredehorst, N. Genov and Ch. Betzel, “Oligomerization of the proteolytic products is an intrinsic property of prion proteins”. Biochem. Biophys. Res. Commun. 323, 1278-1286 (2004).

  13.  D. Georgieva, W. Rypniewski, A. Gabdoulkhakov, N. Genov and Ch. Betzel, „Asp49 phospholipase A2 – elaidoylamide complex: a new mode of inhibition“. Biochem. Biophys. Res. Commun. 319, 1314-1321 (2004).

  14. D. Georgieva, M. Perbandt, W. Rypniewski, K. Hristov, N. Genov and Ch. Betzel, “The X-ray structure of a snake venom Gln48 phospholipase A2 at 1.9 Å resolution reveals anion binding sites“. Biochem. Biophys. Res. Commun. 316, 33-38 (2004).

  15. D. Georgieva, N. Genov, K. Hristov, K. Dierks and Ch. Betzel, „Interactions of the Neurotoxin Vipoxin in Solution Studied by Dynamic Light Scattering“. Biophys. J. 86, 461-466 (2004).

  16. D. Georgieva, N. Genov, M. Perbandt, W. Voelter and Ch. Betzel, „Contribution of disulfide bonds and calcium to the molluscan hemocyanin stability“. Z. Naturforsch. 59c, 281-287 (2004).

  17. D. Georgieva, W. Rypniewski, M. Perbandt, M. Jain, N. Genov and Ch. Betzel, “Crystallization and preliminary X-ray diffraction studies of a toxic phospholipase A2 from the venom of Vipera ammodytes meridionalis complexed to a synthetic inhibitor”. Biochim. Biophys. Acta 1650, 1-3 (2003).

  18. M. Perbandt, I.-H. Tsai, A. Fuchs, S. Banumathi, K. R. Rajashankar, D. Georgieva, N. Kalkura, T. P. Singh, N. Genov and Ch. Betzel, “Structure of the heterodimeric neurotoxic complex Viperotoxin F (RV-4/RV-7) from the venom of Vipera russelli formosensis at 1.9 Å resolution”. Acta Cryst. D59, 1679-1687 (2003).

  19. D. Georgieva, N. Genov, P. Nikolov, B. Aleksiev, K. R. Rajashankar, W. Voelter and Ch. Betzel, “Structure-function relationships in the neurotoxin Vipoxin from the venom of Vipera ammodytes meridionalis”. Spectrochim. Acta A59, 617 – 627 (2003).

  20. S. Windhorst, E. Frank, D. Georgieva, F. Buck, P. Borowski, N. Genov and W. Weber, “The major extracellular protease of the nosocomial pathogen Stenotrophomonas maltophilia: characterization of the protein and molecular cloning of the gene”. J. Biol. Chem. 277, 11042 – 11049 (2002).

  21. D. Georgieva, S. Stoeva, W. Voelter and N. Genov, “Viviparus ater hemocyanin: investigation of the dioxygen-binding site and stability of the oxy- and apo-forms”. Z. Naturforsch. 56c, 843 - 847 (2001).

  22. S. Stoeva, K. Idakieva, D. Georgieva, W. Voelter and N. Genov, “Penaeus monodon (Tiger shrimp) hemocyanin: subunit composition and thermostability”. Z. Naturforsch. 56c, 416 - 422 (2001).

  23. D. Georgieva, S. Stoeva, W. Voelter, N. Genov and Ch. Betzel, “Differences in the specificities of the highly alkalophilic proteinases Savinase and Esperase imposed by changes in the rigidity and geometry of the substrate binding sites”. Arch. Biochem. Biophys. 387, 197 - 201 (2001).

  24. D. Georgieva, S. Stoeva, W. Voelter, N. Genov and Ch. Betzel, “Substrate specificity of the highly alkalophilic bacterial proteinase Esperase®: relation to the X-ray structure”. Curr. Microbiol. (USA) 42, 368 – 371 (2001).

  25. A. Gusterova, D. Spassova, D. Georgieva, R. Tzvetkova and V. Ivanova, “Taxonomy of Thermoactinomyces sacchari, subsp. Nov. No 5, producer of neutral proteinase. I. Phenotypical characteristics”. Compt. Rend. Bulg. Acad. Sci. 53, 83 – 86 (2000).

  26. D. Georgieva, Ch. Betzel, B. Aleksiev and N. Genov, “Spectroscopic investigation of the calcium binding sites in the neurotoxin Vipoxin and its components - relation with the X-ray structure”. Spectrochim. Acta A 56, 2811 - 2816 (2000).

  27. D. Georgieva, S. Stoeva, V. Ivanova, A. Gusterova and W. Voelter, “Specificity of a neutral Zn-dependent proteinase from Thermoactinomyces sacchari toward the oxidized insulin B chain”. Curr. Microbiol. (USA) 41, 70 - 72 (2000).

  28. D. Georgieva, P. Nikolov, V. Ivanova, A. Gusterova and Ch. Betzel, “Fluorescence properties of Savinase®: the X-ray structure in the region of the tryptophyl residues”. Spectrochim. Acta A 55, 2309 - 2319 (1999).

  29. D. Georgieva, N. Genov, K. R. Rajashankar, B. Aleksiev and Ch. Betzel, “Spectroscopic investigation of phenolic groups ionization in the Vipoxin neurotoxic phospholipase A2: comparison with the X-ray structure in the region of the tyrosyl residues”. Spectrochim. Acta A 55, 239 - 244 (1999).

  30.  A. Ali, S. Stoeva, A. Abbasi, D. Georgieva, N. Genov and W. Voelter, “Oxygen transport proteins: II. Chemical and spectroscopic properties of scorpion (Buthus sindicus) native hemocyanin and purified subunit Bsin1”. Comp. Biochem. Physiol. A 122, 65 - 74 (1999).

  31.  P. Dolashka, D. Georgieva, S. Stoeva, N. Genov, R. Rachev, A. Gusterova and W. Voelter, “A novel thermostable neutral proteinase from Saccharomonospora canescence”. Biochim. Biophys. Acta 1382, 207 – 216 (1998).

  32. D. Georgieva, P. Nikolov and Ch. Betzel, “Steady - state and time - resolved fluorescence of Esperase®: comparison with the X-ray structure in the region of the two tryptophans”. Spectrochim. Acta A 54, 1109 - 1116 (1998).

  33.  D. Georgieva, S. Stoeva, S. A. Ali, A. Abbasi, N. Genov and W. Voelter, “Circular dichroism study of the hemocyanin thermostability”. Spectrochim. Acta A 54, 765 - 771 (1998).

  34. N. Genov, I. Goshev, D. Nikolova, D. Georgieva, B. Filippi and I. Svendsen, “A novel thermostable inhibitor of trypsin and subtilisin from the seeds of Brassica nigra: amino acid sequence, inhibitory and spectroscopic properties and thermostability”. Biochim. Biophys. Acta 1341 (2) 157 - 164 (1997).

  35. D. Georgieva and P. Dolashka, “Spectrscopic properties of subtilisin DY selectively modified with fluorescein isothiocyanate”. Spectrochim. Acta A 53, 1515 - 1520 (1997).
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